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Cell 1

Time after Induction

0 200 400 600 800 1000 ms

19min 26 s 19 min 31 s 19min48s 19 min 49.2 s

Figure 10-7 The phage l holin kills without warning. At t = 0, cells tethered to a microscope slide by antiflagellin antibodies were induced for the expression of the plasmid pSI05A52G, which carries the l lysis cassette, including the SA52c early lysis allele, under the control of its cognate promoter, pR0. Single frames chosen from the recordings of a representative cell are depicted here to illustrate the saltatory nature of holin killing. Starting from the time point indicated to the left of the panel, single frames were captured every 200 ms. After induction of the lysis genes, the tethered cells rotate at high and constant speed (first row). About 20 min after induction, rotation of the cell abruptly slows and stops completely within 1-3 (second row). Cell lysis, due to digestion of the cell wall by the l R endolysin, occurs within several seconds after the sudden stop in rotation (third and fourth rows). Adapted from Grundling et al. (54), with permission.

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