Methods

102 healthy mothers were interviewed twice filling a questionnaire assessing the usual habits over the past year (Black G et al. J Am Epidemiol 1986; 124:453-69). That enabled us to identify a group of 86 mothers having at least a portion of fish (200 g) per week (> 28 g/day, fish group) and another group of 16 mothers eating fish never or less than once per week

A sample of mature (> 15 days) breast milk was obtained for measuring total fat, FA pattern (mass spectrometry) vitamin D and 25 D (HPLC and binding protein).

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