Subjects And Methods

The study population consisted of eight mothers feeding a malnourished child. EDTA anti-coagulated blood (2 ml) was taken from the children. Breastmilk samples (5 ml) were collected by manual expression. Fatty acids were determined as their methyl esters by capillary gas chromatography with flame ionisation detection. The milk fatty acid composition of Pakistani mothers was compared with that of mature milk of 25 Dutch mothers. Between-group differences were analysed with Mann Whitney-U test at p<0.05. Correlations of the different polyunsaturated fatty acids (PUFA) between breastmilk and infant RBC were tested with the Spearman test at p<0.05.

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