Amino acid sequence for mouse GCSF receptor76

-25 MVGLGACTLT GVTLIFLLLP RSLES 1 CGHIEISPPV VRLGDPVLAS CTISPNCSKL DQQAKILWRL QDEPIQPGDR 51 QHHLPDGTQE SLITLPHLNY TQAFLFCLVP WEDSVQLLDQ AELHAGYPPA 101 SPSNLSCLMH LTTNSLVCQW EPGPETHLPT SFILKSFRSR ADCQYQGDTI 151 PDCVAKKRQN NCSIPRKNLL LYQYMAIWVQ AENMLGSSES PKLCLDPMDV 201 VKLEPPMLQA LDIGPDWSH QPGCLWLSWK PWKPSEYMEQ ECELRYQPQL 251 KGANWTLVFH LPSSKDQFELCGLHQAPVYT LQMRCIRSSL PGFWSPWSPG 301 LQLRPTMKAP TIRLDTWCQK KQLDPGTVSV QLFWKPTPLQ EDSGQIQGYL 351 LSWNSPDHQG QDIHLCNTTQ LSCIFLLPSE AQNVTLVAYN KAGTSSPTTV...

Maagsittlp Alpedggsga Fppghfkdpk Rlycknggff Lrihpdgrvd 51 Gvreksdphi Klqlqaeerg Wsikgvcan Rylamkedgr Llaskcvtde 101

High molecular weight forms (Mr 24 200, 23 100. 22 500) arise by initiation of translation at CUG triplets coding for Leu residues (bold) at positions 55, 46, and 41,7,M. Truncated form arises by cleavage between amino acids Lys9 Prol0. It is not clear whether these are formed in vivo or are artefacts of purification. Cys residues at positions 34 and 101 are conserved in all members of the FGF family, but do not form disulfide bonds.

Cooh

The G-CSF receptor is a hybrid structure containing an immunoglobulin domain, a haematopoietin domain and three FNIII domains'5,7''. The human receptor exists as two forms varying in their intracytoplasmic domains. One form, 25.1, carries a C kinase phosphorylation site. A potential soluble form of the human receptor has been reported which has deleted the transmembrane region'5. The only murine receptor reported resembles the 25.1 form'6. Human and mouse receptors share 62.5 homology. The...

The Molecule

Apo2L TRAIL is a newly discovered member of the TNF ligand family that is closely related to FasL. Apo2L was initially cloned from human heart and lymphocyte cDNA libraries''2. Apo2L TRAIL is a type II membrane protein, whose C-terminal extracellular domain shows clear homology to other TNF family members. Human Apo2L has been shown to be expressed as a cell surface protein, but a soluble form has also recently been described. The distinct biological function of Apo2L is still unknown, however...

Physicochemical properties of the yIP10 receptor

Amino acids Mr (K) - predicted Potential AMinked glycosylation sites Affinity Kd (m) Binding of human yIP-10 to CXCR3 mediates a Ca flux with an EC50 of 10-20 nM, causes migration of T cells with an ECSo of about 5 nM, and promotes lymphocyte adhesion at subnanomolar concentrations75-76,2. Mouse CXCR3 ligation with ylP-10 causes a Ca flux at 1 (g ml, and mediates T-cell migration at doses over 10ng ml'7. The gene for CXCR3 is on human chromosome Xql3 and on the mouse X chromosome.

The Gh Receptor

The GH receptors is a member of the CKR superfamily but does not possess the WSXWS motif characteristic of this receptor family*. The extracellular domain of the receptor (residues 1-246) produced by proteolysis of membrane hGHR occurs naturally in serum as a soluble GH-binding protein. Structural analysis has shown that one GH molecule binds to two GHR chains. There are two binding sites on opposite faces of the four a-helical GH molecule, each of which binds to essentially the same site on...

Physicochemical properties of IFNy

- expressed'' Potential TV-linked glycosylation sitesc Disulfide bonds a The predicted sequence after removal of a signal peptide of 20 amino acids for human IFNy and 19 amino acids for murine IFNy gives mature proteins of 146 and 136 amino acids respectively. However, sequencing of natural IFNy has shown that the three N-terminal residues (Cys-Tyr-Cys) are removed during post translational modification yielding mature proteins of 143 and 133 amino acids7. b Two species (Mr 25 000 and 20 000)...

Info

MDC binds specifically to CCR4 with high affinity (0.18 nM), mediating a Ca flux with EC50 ranging between 0.1 and 1.0 nM2,3,4. Amino acid sequence for human CCR4 receptor 1 MNPTDIADTT LDESIYSNYY LYESIPKPCT KEGIKAFGEL FLPPLYSLVF 51 VFGLLGNSW VLVLFKYKRL RSMTDVYLLN LAISDLLFVF SLPFWGYYAA 101 DQWVFGLGLC KMISWMYLVG FYSGIFFVML MSIDRYLAIV HAVFSLRART 151 LTYGVITSLA TWSVAVFASL PGFLFSTCYT ERNHTYCKTK YSLNSTTWKV 201 LSSLEINILG LVIPLGIMLF CYSMIIRTLQ HCKNEKKNKA VKMIFAWVL 251 FLGFWTPYNI VLFLETLVEL EVLQDCTFER...

Amino acid sequence for human IL18 receptor

1 ESCTSRPHIT WEGEPFYLK HCSCSLAHEI ETTTKSWYKS SGSQEHVELN 51 PRSSSRIALH DCVLEFWPVE LNDTGSYFFQ MKNYTQKWKL NVIRRNKHSC 101 FTERQVTSKI VEVKKFFQIT CENSYYQTLV NSTSLYKNCK KLLLENNKNP 151 TIKKNAEFED QGYYSCVHFL HHNGKLFNIT KTFNITIVED RSNIVPVLLG 201 PKLNHVAVEL GKNVRLNCSA LLNEEDVIYW MFGEENGSDP NIHEEKEMRI 251 MTPEGKWHAS KVLRIENIGE SNLNVLYNCT VASTGGTDTK SFILVRKADM 301 ADIPGHVFTR GMIIAVLILV AWCLVTVCV IYRVDLVLFY RHLTRRDETL 351 TDGKTYDAFV SYLKECRPEN GEEHTFAVEI LPRVLEKHFG YKLCIFERDV 401 VPGGAWDEI HSLIEKSRRL...

Amino acid sequence for human EGF receptor70

1 LEEKKVCQGT SNKLTQLGTF EDHFLSLQRM FNNCEWLGN LEITYVQRNY 51 DLSFLKTIQE VAGYVLIALN TVERIPLENL QIIRGNMYYE NSYALAVLSN 101 YDANKTGLKE LPMRNLQEIL HGAVRFSNNP ALCNVESIQW RDIVSSDFLS 151 NMSMDFQNHL GSCQKCDPSC PNGSCWGAGE ENCQKLTKII CAQQCSGRCR 201 GKSPSDCCHN QCAAGCTGPR ESDCLVCRKF RDEATCKDTC PPLMLYNPTT 251 YQMDVNPEGK YSFGATCVKK CPRNYWTDH GSCVRACGAD SYEMEEDGVR 301 KCKKCEGPCR KVCNGIGIGE FKDSLSINAT NIKHFKNCTS ISGDLHILPV 351 AFRGDSFTHT PPLDPQELDI LKTVKEITGF LLIQAWPENR TDLHAFENLE 401 IIRGRTKQHG QFSLAWSLN...

Amino acid sequence for mouse CCR7

1 MDPGKPRKNV LWALLVIFQ VCFCQDEVTD DYIGENTTVD YTLYESVCFK 51 KDVRNFKAWF LPLMYSVICF VGLLGNGLVI LTYIYFKRLK TMTDTYLLNL 101 AVADILFLLI LPFWAYSEAK SWIFGVYLCK GIFGIYKLSF FSGMLLLLCI 151 SIDRYVAIVQ AVSRHRHRAR VLLISKLSCV GIWMLALFLS IPELLYSGLQ 201 KNSGEDTLRC SLVSAQVEAL ITIQVAQMVF GFLVPMLAMS FCYLII IRTL 251 LQARNFERNK AIKVIIAWV VFIVFQLPYN GWLAQTVAN FNITNSSCET 301 SKQLNIAYDV TYSLASVRCC VNPFLYAFIG VKFRSDLFKL FKDLGCLSQE 351 RLRHWSSCRH VRNASVSMEA ETTTTFSP ' Hedrick, J.A. and Zlotnik, A. (1997) J. Immunol. 159,...

The Receptor Cxcr3

The yIP-10 receptor CXCR3, also known as GPR9, is a high-affinity functional receptor for yIP-10 mediating chemotaxis and adhesion of Th 1 lymphocytes and NK cells'577. CXCR3 is also receptor for three other CXC chemokines I-TAC, MIG and 6Ckine76'7i'79. Eotaxin may act as a natural receptor antagonist for CXCR320. yIP-10 has also been shown to be an inverse agonist for the herpesvirus 8 G proteincoupled receptor (accession code P88966), acting to block the constituitive signalling mediated by...

The Angiostatin Receptor

The angiostatin receptor remains to be identified. References ' Sim, B.K. et al. (1997) Cancer Res. 57, 1329-1334. 2 O Reilly, M.S. et al. (1994) Cell 79, 315-328. 3 Gately, S. et al. (1996) Cancer Res. 56, 4887 1890. 4 Gately, S. et al. (1997) Proc. Natl Acad. Sci. USA 94, 10868-10872. 5 O Reilly, M.S. et al. (1996) Nature Med. 2, 689-692. 6 Ji, W.R. et al. (1998) FASEB J. 12, 1731-1738. 7 Cao, Y. et al. (1996) J. Biol. Chem. 271, 29461-29467. 8 Luo, J. et al. (1998) Biochem. Biophys. Res....

Amino acid sequence for human Epo receptor70

1 APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP 51 GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV 101 TAASGAPRYH RVIHINEWL LDAPVGLVAR LADESGHWL RWLPPPETPM 151 TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM 201 AEPSFGGFtVS AWSEPVSLLT PSDLDPLILT LSLILWILV LLTVLALLSH 251 RRALKQKIWP GIPSPESEFE GLFTTHKGNF QLWLYQNDGC LWWSPCTPFT 301 EDPPASLEVL SERCWGTMQA VEPGTDDEGP LLEPVGSEHA QDTYLVLDKW 351 LLPRNPPSED LPGPGGSVDI VAMDEGSEAS SCSSALASKP SPEGASAASF 401 EYTILDPSSQLLRPWTLCPE...

The Gro Family Receptor Cxcr2

The GRO family have been shown to bind to the CXCR2 receptor with high affinity7,9 (see IL-8 entry (page 80) for full details). 1 Oppenheim, J.J. et al. (1991) Annu. Rev. Immunol. 9, 617-648. 2 Haskill, S. et al. (1990) Proc. Natl Acad. Sci. USA 87. 7732-7736. 3 Sager, R. et al. (1991) Adv. Exp. Med. Biol. 305, 73-77. 4 Aniscowicz, A. et al. (1987) Proc. Natl Acad. Sci. USA 84, 7188-7192. 5 Tekamp-Olson, P. et al. (1990) J. Exp. Med. 172, 911-919. 6 Oquendo, P. et al. (1989) J. Biol. Chem. 264,...

The Il15 Receptor

IL-15 uses two distinct receptor systems. On T cells and NK cells, the type IIL-15R system is used. This includes IL-2 15RP, which is shared with IL-2, IL-4, IL-7 and IL-9, the vc subunit and an IL-15-specific receptor subunit, IL-15RaJ,6-i. In addition, mast cells utilize a novel 60-65-kDa IL-15RX subunit which is unrelated to the IL-2R system. This is referred to as the type IIIL-15R system9 IL-15Ra binds IL-15 with a very high affinity (Kd 1011 m). In the absence of IL-15R< x , IL-2 15RP...

THE Apo2Ltrail Receptor

Apo2L TRAIL interacts with four human cell surface receptors that form a distinct group within the TNFRSF (TRAIL-R1-TRAIL-R4) (for a review see ref. 10). TRAIL-R1, also called DR4 (death receptor 4) and TRAIL-R2 (also called DR5 (death receptor 5 and TRICK2 or KILLER)72 have death domains (see FasL entry (page 225)) that signal apoptosis. TRAIL-R3 (also called DcRl (decoy receptor 1, TRID or LIT) is a GPI-linked cell surface protein that lacks a cytoplasmic domain and consequently a functional...

Amino acid sequence for human CD27

1 TPAPKSCPER HYWAQGKLCC QMCEPGTFLV KDCDQHRKAA QCDPCIPGVS 51 FSPDHHTRPH CESCRHCNSG LLVRNCTITA NAECACRNGW QCRDKECTEC 101 DPLPNPSLTA RSSQALSPHP QPTHLPYVSE MLEARTAGHM QTLADFRQLP 151 ARTLSTHWPP QRSLCSSDFI RILVIFSGMF LVFTLAGALF LHQRRKYRSN 201 KGESPVEPAE PCRYSCPREE EGSTIPIQED YRKPEPACSP Mature CD27 is formed after removal of the signal peptide. Conflict in sequence A-> T at position 59 of precursor sequence.

Tqlpydhkwe Fprnrlsfgk Tlgagafgkv Veatayglik Sdaamtvavk 601 Mlkpsahlte Realmselkv Lsylgnhmni Vnllgactvg Gptlviteyc 651

Tyr799 is site of autophosphorylation. References 2 Morrison-Graham, K. and Takahashi, Y. (1993) BioEssays 15, 77-83. 3 Flanagan, J.G. et al. (1991) Cell 64, 1025-1035. 4 Anderson, D.M. et al. (1991) Cell Growth Differ. 2, 373-378. 5 Arakawa, T. et al. (1991) J. Biol. Chem. 266, 18942-18948. 6 Martin, F.H. et al. (1990) Cell 63. 203-211. 7 Anderson, D.M. et al. (1990) Cell 63, 235-243. s Zsebo, K.M. et al. (1990) Cell 63, 213-224. 9 Huang, E. et al. (1990) Cell 63, 225-233. 10 Lu, H.S. et al....

The Baff Receptor

B-cell maturation antigen (BCMA) was first identified as part of a translocation event in a malignant T-cell lymphoma patient7. Subsequent identification of the mouse BCMA gene and further motif analysis led to the prediction that BCMA is a member of the TNF receptor superfamily* Recently it has been shown that BCMA is the receptor for BAFF9. Characterization of BCMA showed that it is primarily expressed in immune organs and mature B-cell lines7,,,> . TNF receptor family members are generally...

The Tslp Receptor

TSLP binds to a receptor complex of the TLSR receptor and the IL-7R a-chain. The common y-chain is not required for high-affinity binding of TSLP or signalling. The TLSR receptor is a type 1 transmembrane protein belonging to the haematopoietin receptor superfamily but it lacks the second of the four conserved cysteine residues present in other members of this family and instead of the characteristic WSXWS motif it has WTAVT5'6. It has significant homology with the common y-chain6....

Llaqgkivki Cdfglardim Hdsndvskgs Tflpvkwmap Esifdnlytt 851 Lsdvwsygil Lweifslggt Pypgmmvdst Fynkiksgyr Makpdhatse 901

The extracellular Cys residues form disulfide bonds in Ig-SF domains and are conserved in both PDGF receptors and other members of the split tyrosine kinase family (c-kit, M-CSFR). Tyr707, 718, 738, 969 and 994 are autophosphorylated (by similarity with the human 3-receptor) and are involved in binding to SH2 domains of signalling proteins (see above under signal transduction)2. Amino acid sequence for mouse PDGFR-p2*

Amino acid sequence for human leptin receptor ObR

1 MICQKFCWL LHWEFIYVIT AFNLSYPITP WRFKLSCMPP NSTYDYFLLP 51 AGLSKNTSNS NGHYETAVEP KFNSSGTHFS NLSKTTFHCC FRSEQDRNCS 101 LCADNIEGKT FVSTVNSLVF QQIDANWNIQ CWLKGDLKLF ICYVESLFKN 151 LFRNYNYKVH LLYVLPEVLE DSPLVPQKGS FQMVHCNCSV HECCECLVPV 201 PTAKLNDTLL MCLKITSGGV IFQSPLMSVQ PINMVKPDPP LGLHMEITDD 251 GNLKISWSSP PLVPFPLQYQ VKYSENSTTV IREADKIVSA TSLLVDSILP 301 GSSYEVQVRG KRLDGPGIWS DWSTPRVFTT QDVIYFPPKI LTSVGSNVSF 351 HCIYKKENKI VPSKEIVWWM NLAEKIPQSQ YDWSDHVSK VTFFNLNETK 401 PRGKFTYDAV YCCNEHECHH...

Amino acid sequence for human IL17 receptor7

1 SLRLLDHRAL VCSQPGLNCT VKNSTCLDDS WIHPRNLTPS SPKDLQIQLH 51 FAHTQQGDLF PVAHIEWTLQ TDASILYLEG AELSVLQLNT NERLCVRFEF 101 LSKLRHHHRR WRFTFSHFW DPDQEYEVTV HHLPKPIPDG DPNHQSKNFL 151 VPDCEHARMK VTTPCMSSGS LWDPNITVET LEAHQLRVSF TLWNESTHYQ 201 ILLTSFPHME NHSCFEHMHH IPAPRPEEFH QRSNVTLTLR NLKGCCRHQV 251 QIQPFFSSCL NDCLRHSATV SCPEMPDTPE PIPDYMPLWV YWFITGISIL 301 LVGSVILLIV CMTWRLAGPG SEKYSDDTKY TDGLPAADLI PPPLKPRKVW 351 11YSADHPLY VDVVLKFAQF LLTACGTEVA LDLLEEQAIS EAGVMTWVGR 401 QKQEMVESNS KIIVLCSRGT...

Amino acid sequence for mouse activin receptor type II

1 SGRGEAETRE CIYYNANWEL ERTNQSGLER CEGEQDKRLH CYASWRNSSG 51 TIELVKKGCW LDDFNCYDRQ ECVATEENPQ VYFCCCEGNF CNERFTHLPE 101 PGGPEVTYEP PPTAPTLLTV LAYSLLPIGG LSLIVLLAFW MYRHRKPPYG 151 HVDIHEVRQC QRWAGRRDGC ADSFKPLPFQ DPGPPPPSPL VGLKPLQLLE 201 IKARGRFGCV WKAQLMNDFV AVKIFPLQDK QSWQSEREIF STPGMKHENL 251 LQFIAAEKRG SNLEVELWLI TAFHDKGSLT DYLKGNIITW NELCHVAETM 301 SRGLSYLHED VPWCRGEGHK PSIAHRDFKS KNVLLKSDLT AVLADFGLAV 351 RFEPGKPPGD THGQVGTRRY MAPEVLEGAI NFQRDAFLRI DMYAMGLVLW 401 ELVSRCKAAD GPVDEYMLPF...

Physicochemical properties of betacellulin

Amino acids - precursor 178 177 Mr (K) - predicted 8.98 9.052 Potential iV-linked glycosylation sites 1 3 a This represents the predicted pi of human and mouse betacellulin. b Both human and mouse betacellulin contain putative signal peptides (residues 1-31) in addition to propeptides (residues 112-178 (human) and 112-177 (mouse)) which are removed in the mature molecules.

Vtnqdlpvik Cvlishenng Ssagkpssyr Mvrgspedlq Ctprrqsegt 51 Vyeaatveva Esgsitlqvq Latpgdlscl Wvfkhsslgc Qphfdlqnrg 101

Tyr947 is required for association with GRB2 and Tyr933 with PI-3 kinase. Protein kinase domain amino acids 584-919. Note The sequence given above is the confirmed sequence for murine Flt3 originally reported by Rosnet et al.' . The SwissProt sequence is that of Flk2 reported by Matthews et al. This sequence differs from the Flt3 sequence at several positions and by a number of C-terminal amino acids but it has never been confirmed and the validity of the Flk2 sequence is unclear.

Vrssgvpgl Teaektvtds Se

CCR8 (also known as TER-1, ChemRl, GPRCY6 or CKRL1) is expressed on T cells and NK cells, and also binds to TARC and MIP-ip '5. CCR8 is also a receptor for viral chemokine antagonists including the Kaposi-associated herpesvirus (HHV-8) proteins viral MIP-1 and viral MIP-Il'6 and the molluscum con-tagiosum poxvirus protein MC148'7. See the TARC entry (page 457).

Amino acid sequence for human DcR3

-2 9 MRALEGPGLS LLCLVLALPA LLPVPAVRG 1 VAETPTYPWR DAETGERLVC AQCPPGTFVQ RPCRRDSPTT CGPCPPRHYT 51 QFWNYLERCR YCNVLCGERE EEARACHATH NRACRCRTGF FAHAGFCLEH 101 ASCPPGAGVI APGTPSQNTQ CQPCPPGTFS ASSSSSEQCQ PHRNCTALGL 151 ALNVPGSSSH DTLCTSCTGF PLSTRVPGAE ECERAVIDFV AFQDISIKRL 2 01 QRLLQALEAP EGWGPTPRAG RAALQLKLRR RLTELLGAQD GALLVRLLQA 7 Suda, T. et al. (1993) Cell 75, 1169-1178. 2 Lynch, D.H. et al. (1995) Immunol. Today 16, 569-574. 3 Lynch, D.H. et al. (1994) Immunity 1, 131-136. 4 Takahashi, T. et...

Physiochemical properties of the CD30L

Amino acids - precursor 595 480 Mr (K) - predicted 63.74 53.21 Potential A'-linked glycosylation sites 2 Affinity Kd (m) 9 2.5 x 109 a After removal of predicted signal peptide. Signal transduction CD30L-CD30 interactions lead to activation of NFkB at levels similar to that observed for TNFa'5. The cytosolic domain of CD30, like that of TNFR II and CD40, interacts with TNF receptor-associated factors . Traf-2 plays a key role in transducing signals from CD30 to NFkB activation'5. The region of...

Interactions Between Cytokines

Individually, cytokines are potent molecules which, in vitro, can cause changes in cell proliferation, differentiation and movement at nanomolar to picomolar concentrations. Injection of cytokines into animals and humans either systemically or locally can also have profound effects on leukocyte migration and function, haematopoietic cell numbers, temperature regulation, acute phase responses, tissue remodelling and cell survival. The individual entries in this book describe the specific...

Amino acid sequence for mouse activin receptor type I

1 VEDEKPKVNQ KLYMCVCEGL SCGNEDHCEG QQCFSSLSIY DGFHVYQKGC 51 FQVYEQGKMT CKTPPSPGQA VECCQGDWCN RNITAQLPTK GKSFPGTQNF 101 HLEVGLIILS WFAVCLLAC ILGVALRKFK RRNQERLNPR DVEYGTIEGL 151 ITTNVGDSTL AELLDHSCTS GSGSGLPFLV QRTVARQITL LECVGKGRYG 201 EVWRGSWQGE NVAVKIFSSR DEKSWFRETE LYNTVMLRHE NILGFIASDM 251 TSRHSSTQLW LITHYHEMGS LYDYLQLTTL DTVSCLRIVL SIASGLAHLH 301 IEIFGTQGKS AIAHRDLKSK NILVKKNGQC CIADLGLAVM HSQSTNQLDV 351 GNNPRVGTKR YMAPEVLDET IQVDCFDSYK RVDIWAFGLV LWEVARRMVS 401 NGIVEDYKPP FYDWPNDPS...

Amino acid sequence for mouse FGFR42

1 LEASEEMEQE PCLAPILEQQ EQVLTVALGQ PVRLCCGRTE RGRHWYKEGS 51 RLASAGRVRG WRGRLEIASF LPEDAGRYLC LARGSMTWH NLTLLMDDSL 101 TSISNDEDPK TLSSSSSGHV YPQQAPYWTH PQRMEKKLHA VPAGNTVKFR 151 CPACRNPMPT IHWLKDGQAF HGENRIGGIR LRHQHWSLVM ESWPSDRGT 201 YTCLVENSLG SIRYSYLLDV LERSPHRPIL QAGLPANTTA WGSDVELLC 251 KVYSDAQPHI QWLKHWING SSFGADGFPY VQVLKTTDIN ISEVQVLYLR 301 NVSAEDAGEY TCLAGNSIGL SYQSAWLTVL PEEDLTWTTA TPEARYTDI_I 351 LYVSGSLVLL VLLLLAGVYH RQVIRGHYSR QPVTIQKLSR FPLARQFSLE 401 SRSSGKSSLS LVRGVRLSSS...

Amino acid sequence for human BMP2

1 MVAGTRCLLA LLLPQVLLGG AAGLVPELGR RKFAAASSGR PSSQPSDEVL 51 SEFELRLLSM FGLKQRPTPS RDAWPPYML DLYRRHSGQP GSPAPDHRLE 101 RAASRANTVR SFHHEESLEE LPETSGKTTR RFFFNLSSIP TEEFITSAEL 151 QVFREQMQDA LGtmSSFHHR INIYEIIKPA TANSKFPVTR LLDTRLVNQtH 201 ASRWESFDVT PAVMRWTAQG HANHGFWEV AHLEEKQGVS KRHVRISRSL 251 HQDEHSWSQI RPLLVTFGHD GKGHPLHKRE A'i QAKHKQRK RLKSSCKRHP 301 LYVDFSDVGW NDWIVAPPGY HAFYCHGECP FPLADHLNST NHAIVQTLVN 351 SVNSKIPKAC CVPTELSAIS MLYLDENEKV VLKNYQDMW EGCGCR BMP-2 has a potential signal...

Physicochemical properties of TRANCE

Amino acids - precursor 317 316 - matureft 317 177 316 177 Mr (K) - predicted 35.47 35.4 Potential A-linked glycosylation sites 2 2 b Human and mouse TRANCE lack signal peptides, however a 177-amino-acid form is shed. The crystal structure for TRANCE is unknown, but is predicted to contain all 10 (3-sheaths in its extracellular domain, which are folded into an antiparallel P-sandwich structure with a 'jelly roll' topology (three monomers associating about a 3-fold axis of symmetry forming a...

The Mig Receptor Cxcr3

MIG shares a receptor CXCR3 with two other chemokines, yIP-10 and I-TAC9. See yIP-10 entry (page 328). MIG binds to CXCR3 with high affinity, although precise quantitation of Kd was impossible due to high nonspecific binding to cell surface proteoglycans9. MIG causes a calcium flux with an EC50 of about 100nM in IL-2-activated peripheral blood lymphocytes, but promotes adhesion to endothelial cells at subnanomolar concentrations*. ' Farber, J.M. et al. (1990) Proc. Natl Acad. Sci. USA 87,...

Apviepsgpe Lwepgetvt Lrcvsngsve Wdgpispiwt Ldpespgstl 51 Ttsnatfknt Gtyrcteled Pmagsttihl Yvkdpahswn Llaqevtwe 101

Tyr678, 687 and 788 are autophosphorylated. References ' Sherr, C.J. and Stanley, E.R. (1990) In Growth Factors, Differentiation Factors and Cytokines, Habenicht, A. ed Springer-Verlag, Berlin. 2 Kawasaki, E.S. et al. (1985) Science 230, 291-296. 3 Wong, G.C. et al. (1987) Science 235, 1504-1508. 4 Ladner, M B. et al. (1988) Proc. Natl Acad. Sci. USA 85, 6706-6710. 5 Ladner, M.B. et al. (1987) EMBO J. 6, 2693-2698. 6 Cerreti, D.P. et al. (1988) Mol. Immunol. 25, 761-770. 7 Testa, N.G. et al....

Physicochemical properties of IL17

Amino acids - precursor 155 158 Mr (K) - predicted 15.1 15.0 Potential N-linked glycosylation sites 1' 1' Disulfide bonds a After removal of signal peptide determined by N-terminal sequencing2. b Corresponding to glycosylated and non-glycosylated homodimers2. c Conserved in hIL-17, mIL-17 and vIL-17. d There are six conserved Cys residues in human, mouse and viral IL-17.

The Ox4ql Receptor

The OX-40L receptor (OX-40R) is a cell surface glycoprotein of the tumour necrosis factor receptor family that is expressed primarily on activated CD4+ T cells7. Engagement of OX-4 R by the OX-40 ligand (OX-40L) is known to costimulate the production of cytokines by activated T lymphocytes* and to rescue effector T cells from activation-induced cell death. OX-40-deficient mice have greatly diminished T helper cell responses. Accumulating evidence now favours a crucial role for OX-40L OX-40...

THE MlPla Receptors Ccr1 And Ccr5

The two main receptors that mediate the biological activities of MlP-la are CCR1 and CCR5, both of which are broadly expressed. CCR4 and CCR9 D6 have also been reported as receptors for MlP-la, but their physiological relevance as MlP-la receptors is questionable. CCR1 was originally cloned following a homology hybridization cloning approach from human leukocytes'5''*. The cloned receptor expressed in human kidney cells binds MlP-la (Kd 5 mn) and RANTES (Kd 468 nivi) with high affinity and...

Amino acid sequence for human APRIL2

1 MPASSPFLLA PKGPPGNMGG PVREPALSVA LWLSWGAALG AVACAMALLT 51 QQTELQSLRR EVSRLQGTGG PSQNGEGYPW QSLPEQSSDA LEAWENGERS 101 RKRRAVLTQK QKKQHSVLHL VPINATSKDD SDVTEVMWQP ALRRGRGLQA 151 QGYGVRIQDA GVYLLYSQVL FQDVTFTMGQ WSREGQGRQ ETLFRCIRSM 201 PSHPDRAYNS CYSAGVFHLH QGDILSVIIP RARAKLNLSP HGTFLGFVKL Human APRIL contains a single TV-linked glycosylation site (Asnl24) within the receptor-binding motif. Traditional cDNA library screening has also identified two additional splice variants, designated TRDL-ip...

Qhlgmtkcei Mcdkmtsrip Valliryqln Qescgkraiv Lettqhrrfc 51 Adpkekwvqd Amkhldhqaa Altknggkfe Krvdnvtpgi Tlatrglsps 101

Dibasic RR cleavage site (which generates soluble fractalkine) is shown in bold. The specific fractalkine receptor CX3R1, also known as V28 or CMKBRL1, is a typical 7-TM spanning chemokine receptor5'7-9. CX3R1 binds to fractalkine with high affinity, mediating a calcium flux which is pertussis toxin sensitive, and chemotaxis. Binding of the membrane form of fractalkine to cells bearing the receptor mediates strong adhesion, which is not dependent on G protein...

Physicochemical properties of RANTES

Amino acids - precursor 91 91 Potential A-linked glycosylation sites 0 0 a Dipeptidylpeptidase IV truncated form of RANTES lacking residues 1-2 retains CCR5 binding but loses CCR1 activity. b Possibly some O-linked glycosylation Each monomer consists of a three-stranded antiparallel P-sheet in a Greek key motif with a C-terminal helix packed across the sheet, an arrangement similar to the monomeric structure of other members of this chemokine family (IL-8, PF-4, MGSA Groa and MIP-1P). Overall,...

Mdytmepnvt Mtdyypdfft Apcdaefllr Gsmlylaily Cvlfvlgllg 51 Nslvilvlvg Ckklrsitdi Yllnlaasdl Lfvlsipfqt Hnlldqwvfg 101

References ' Brown, K.D. et al. (1989) J. Immunol. 142. 679-687. 2 Wilson, S.D. et al. (1990) J. Immunol. 145, 2745-2744. 3 Burd, P.R. et al. (1987) J. Immunol. 139, 3126-3131. 4 Schall, T.J. (1991) Cytokine 3, 165-183. 5 Miller, M.D. et al. (1992) Proc. Natl Acad. Sci. USA 89. 2950-2954. 6 D Ambrosio, D. et al. (1988) J. Immunol. 161,5111-5115. 7 Burd. P.R. et al. (1989) J. Exp. Med. 170, 245-257. 8 Wilson, S.D. et al. (1990) J. Exp. Med. 171, 1301-1314. 9...

Mmdiyvclkr Pswmvdnkrm Rtasnfqwll Stfillylmn Qvns

1 QKKGAPHDLK CVTNNLQVWN CSWKAPSGTG RGTDYEVCIE NRSRSCYQLE 51 KTSIKIPALS HGDYEITINS LHDFGSSTSK FTLNEQNVSL IPDTPEILNL 101 SADFSTSTLY LKWNDRGSVF PHRSNVIWEI KVLRKESMEL VKLVTHNTTL 151 NGKDTLHHWS WASDMPLECA IHFVEIRCYI DNLHFSGLEE WSDWSPVKNN 201 SWIPDSQTKV FPQDKVILVG SDITFCCVSQ EKVLSALIGH TNCPLIHLDG 251 ENVAIKIRNI SVSASSGTNV VFTTEDNIFG TVIFAGYPPD TPQQLNCETH 301 DLKEIICSWN PGRVTALVGP RATSYTLVES FSGKYVRLKR AEAPTNESYQ 351 LLFQMLPNQE IYNFTLNAHN PLGRSQSTIL VNITEKVYPH TPTSFKVKDI 401 NSTAVKLSWH LPGNFAKINF...

Physicochemical properties of APRIL

- mature'' Mr (K) - predicted - expressed Potential AMinked glycosylation sites Disulfide bonds a This represents the predicted pi of APRIL TRDL-lx 3-D structure The crystal structure for APRIL is unknown, but is predicted to contain eight (3-strands in its extracellular domain, which are folded into an antiparallel (3-sandwich structure with a 'jelly-roll' topology (three monomers associating about a 3-fold axis of symmetry forming a bell-shaped trimer). based on its homology with lymphotoxin...

Other Symbols Used

V-glycosylation sites O-linked glycosylation Glycosoaminoglycan Leucine zipper (LZ) Figure 1 Models for domains and repeats found in leukocyte membrane proteins. These models are used in the diagrams drawn for the entries in Section II. The chromosomal location of the receptors is taken from original papers and or HGM 1 4,s. Amino acid sequences of cytokine receptors Sequence data for human and mouse receptors are given as described above for the cytokines. Transmembrane domains are underlined...

Amino acid sequence for mouse GDNF

1 FPLPAGKRLL EAPAEDHSLG HRRVPFALTS DSNMPEDYPD QFDDVMDFIQ 51 ATIKRLKRSP DKQAAALPRR ERNRQAAAAS PENSRGKGRR GQRGKNRGCV 101 LTAIHLNVTD LGLGYETKEE LIFRYCSGSC ESAETMYDKI LKNLSRSRRL 151 TSDKVGQACC RPVAFDDDLS FLDDNLVYHI LRKHSAKRCG CI The propeptide sequence (20-77) shown in italics is removed to generate the mature protein. Disulfide bridges are formed between Cysl 18-179, 145-208, 149-210 by similarity and an interchain disulfide ridge is predicted using Cysl78. Two isoforms of mouse GDNF are produced...

Amino acid sequence for human 41BB6

1 FERTRSLQDP CSNCPAGTFC DNNRNQICSP CPPNSFSSAG GQRTCDICRQ 51 CKGVFRTRKE CSSTSNAECD CTPGFHCLGA GCSMCEQDCK QGQELTKKGC 101 KDCCFGTFND QKRGICRPWT NCSLDGKSVL VNGTKERDW CGPSPADLSP 151 GASSVTPPAP AREPGHSPQI ISFFLALTST ALLFLLFFLT LRFSWKRGR 201 KKLLYIFKQPFMRPVQTTQE EDGCSCRFPEEEEGGCEL The transmembrane region is underlined. There is a conflict in the sequence at position 107 K- R.

The Molecules

Platelet factor 4 (PF-4) is an alpha granule protein released by platelets with wide biological activities. PF-4 is normally bound to a 53-kDa carrier proteoglycan7. It binds to and neutralizes heparin, is a potent anti-angiogenic factor in vivo, it can suppress or enhance haematopoietic progenitor cell survival, activates basophils and mast cells to release histamine, stimulates neutrophil adherence to endothelium, is chemotactic for fibroblasts, and promotes monocyte survival2-7. There is no...

Spygsdttpccfaylslelprahvkeyfyt Sskcsnlaw Fvtrrnrqvc 51 Anpekkwvqeyinylems

THE RANTES RECEPTORS CCR1, CCR3, CCR5 and CCR9 RANTES binds to the CCR1, CCR3, CCR5 and CCR9 receptors, mediating chemotaxis and activation of the various cell types bearing these receptors, lymphocytes, monocytes, eosinophils and basophils. CCR5 has also been shown to be an important co-receptor for HIV infection, with numerous polymorphisms in the coding and noncoding regions of the gene affecting HIV infection762''. For a full description of the CCR1 receptor see the MIP-la entry (page 384),...

Mmcqkfywl Lhweflyvia Alnlaypisp Wkfklfcgpp Nttddsflsp 51 Agapnnasal Kgaseaivea Kfnssgiyvp Elsktvfhcc Fgneqgqncs 101

A - PETFE -> RTDTL at position 890-894 terminating at 894 (895-1162 missing). B - full-length form sequence shown here. C - PET-> VTV at position 890-892 terminating at 892 (893-1162 missing) D - PETFEHLFTKH-> DISFHEVFIFR at position 890-900 terminating at 900 (901-1162 missing). E - DNFIPIEK-> GMCTVLFM at position 797-804 terminating at 804 (805-1162 missing) has no transmembrane domain and is released from the cell. ' Friedman, J.M. and Halaas, J.L. (1998) Nature 395, 763-770. 2...

The Protein Tyrosine Kinase Receptor Superfamily

Receptor tyrosine kinases all have a large glycosylated extracellular ligand-binding domain, a single transmembrane domain and an intracellular tyrosine kinase catalytic domain63,6''. This superfamily of receptors can be divided into four subclasses. Subclass I receptors are monomeric with two extracellular cysteine-rich repeat sequences and include the EGFR. Disulfide-linked heterotetrameric (a2(32) structures with similar cysteine-rich sequences are found in subclass II receptors such as...

The Isg15 Receptor

Chem. 262, 11315-11323. 2 D Cunha, J. et al. (1996) J. Immunol. 157. 4100-4108. 3 Reich, N. et al. (1987) Proc. Natl Acad. Sci. USA 84, 6394-6398. 4 D Cunha, J. et al. (1996) Proc. Natl Acad. Sci. USA 93, 211-215. 5 Knight, E. and Cordova, B.J. (1991) Immunology 146, 2280-2284. 6 Knight, E. et al. (1988) J. Biol. Chem. 263, 4520 522. 7 Blomstrom, D.C. et al. (1986) J. Biol. Chem. 261, 8811-8816.

Amino acid sequence for mouse IL16 propeptide

1 MDYSFDITAE DPWVRISDCI KNLFSPIMSE NHSHRPLQPN TSLGEEDGTQ 51 GCPEGGLSKM DAANGAPRVY KSADGSTVKK GPPVAPKPAW FRQSLKGLRN 101 RAPDPRRPPE VASAIQPTPV SRDPPGPQPQ ASSSIRQRIS SFENFGSSQL 151 PDRGVQRLSL QPSSGETTKF PGKQDGGRFS GLLGQGATVT AKHRQTEVES 201 MSTTFPNSSE VRDPGLPESP PPGQRPSTKA LSPDPLLRLL TTQSEDTQGP 251 GLKMPSQRAR SFPLTRTQSC ETKLLDEKAS KLYSISSQLS SAVMKSLLCL 301 PSSVSCGQIT CIPKERVSPK SPCNNSSAAE GFGEAMASDT GFSLNLSELR 401 KQLDSIHVTI LHKEEGAGLG FSLAGGADLE NKVITVHRVF PNGLASQEGT 451 IQKGNEVLSI NGKSLKGATH...

The Cytokine Facts Book

Department of Biochemistry & Biotechnology Institute, Trinity College, Dublin, Ireland Department of Biochemistry & Biotechnology Institute, Trinity College, Dublin, Ireland Biocomm International, Melbourne, Australia Institute of Child Health, University College London, London, UK

Physicochemical properties of the eotaxin 1 receptor CCR3

Amino acids - precursor 355 359 Mr (K) - predicted 41.0 41.8 Potential V-linked glycosylation sites 0 0 Affinity Kd (m) 1x10 1om Western blotting of the human CCR3 reveals several bands between 45 and 55 kDa, possibly O-glycosylated forms. Binding of eotaxin 1 to cells expressing CCR3 causes a pertussis toxin inhibitable calcium flux, with an ED50 of 0.3 iim 14. The human gene is located on chromosome 3 7.

The Elc Receptor Ccr7

The ELC receptor, CCR7, is shared with the related 6Ckine chemokine. For a full description of the receptor, see the 6Ckine entry (page 188). ELC binds to CCR7 with high affinity, causing a pertussis toxin-sensitive Ca2+ flux with an ED50 of 0.93.0 nm, and stimulating chemotaxis with an ED50 of about 100 iim''6'. ' Yoshida, R. et al. (1997) J. Biol. Chem. 272, 13803-13809. 2 Rossi, D.L. et al. (1997) J. Immunol. 158, 1033-1036. 3 Yoshida, R. et al. (1998) J. Exp. Med. 188, 181-191. 4 Kim, C.H....

Iypsgviglv Phlgdrekrd Svcpqgkyih Pqnnsicctk Chkgtylynd 51 Cpgpgqdtdc Recesgsfta Senhlrhcls Cskcrkemgq Veissctvdr 101

Conflicting sequence Pro391 missing, GPAA-> APP at positions 422-425. Soluble receptor amino acids 20-180. Amino acid sequence for human TNF type II (p75) receptor27 1 LPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD 51 TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP 101 GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDWCK PCAPGTFSNT 151 TSSTDICRPH QICNWAIPG NASMDAVCTS TSPTRSMAPG AVHLPQPVST 201 RSQHTQPTPE PSTAPSTSFL LPMGPSPPAE GSTGDFALPV GLIVGVTALG 251 LLIIGWNCV IMTQVKKKPL CLQREAKVPH...

Isbn 0121551423

Library of Congress Catalog Number 2001092474 A catalogue record for this book is available from the British Library INSTRUCTIONS ON GAINING ACCESS TO THE ON-LINE VERSION OF THE CYTOKINE FACTSBOOK, SECOND EDITION Access for a limited period to an on-line version of The Cytokine FactsBook, Second Edition is included in the purchase price of the print edition. The on-line version has been uniquely and persistently identified by the Digital Object Identifier (DOI) from any Web Browser, buyers of...

Amino acid sequence for mouse angiostatin

1 MDHKEVILLF LLLLKPGQGD SLDGYISTQG ASLFSLTKKQ LAAGGVSDCL 51 AKCEGETDFV CRSFQYHSKE QQCVIMAENS KTSSIIRMRD VILFEKRI yL 101 SECKTGIGNG YRGTMSRTKS GVACQKWGAT FPHVPNYSPS THPNEGLEEN 151 YCRNPDNDEQ GPWCYTTDPD KRYDYCNIPE CEEECMYCSG EKYEGKISKT 201 MSGLDCQAWD SQSPHAHGYI PAKFPSKNLK MNYCHNPDGE PRPWCFTTDP 251 TKRWEYCDIP RCTTPPPPPS PTYQCLKGRG ENYRGTVSVT VSGKTCQRWS 301 EQTPHRHNRT PENFPCKNLE ENYCRNPDGE TAPWCYTTDS QLRWEYCEIP 351 SCESSASPDQ SDSSVPPEEQ TPWQECYQS DGQSYRGTSS TTITGKKCQS 4 01 WAAMFPHRHS KTPENFPDAG...

The Ct1 Receptor

The receptor for CT-1 has been partially characterized. Several studies suggest that CT-1 binds to and activates the LIF receptor. More recent studies have confirmed this observation and demonstrate a Kd of 2 nM for CT-1 binding to gp 190, which is identical to that observed for LIF binding. gpl30 acts as a high-affinity converter for CT-1 binding to gpl90. For details of the LIF receptor see the LIF entry (page 346) and for the gpl30 subunit see the IL-6 entry (page 69). The existence of a...

The Haematopoietic Receptor Superfamily

This is the largest cytokine receptor superfamily by far and includes IL-2R (3- and y-chains, IL-4R, IL-3R a- and (3-chains, IL-5R a- and p-chains, IL-6R, gpl30. IL-9R, IL-12R, G-CSFR, GM-CSFR, CNTFR, LIFR, EpoR, PRLR and GHR. The IL-7R is also usually included in this family but the case for its inclusion is weak. It has a very low ALIGN score2 and few of the conserved amino acids in the domain'. Domain structure of the haematopoietic receptor superfamily The extracellular regions of the...

The Osm Receptor

High- and low-affinity receptors for OSM have been detected in a wide variety of cell types. The low-affinity receptor for OSM has been identified as gpl30, a molecule that forms a component of the IL-6 and LIF receptor complex (see IL-6 entry (page 69) and LIF entry (page 346)). Receptor binding in this family of receptors is characterized by low-affinity binding to an a receptor which is converted to a high-affinity complex due to a second unrelated P receptor. gpl30 is the high-affinity...

Amino acid sequence for human CCR7

1 MDLGKPMKSV LWALLVIFQ VCLCQDEVTD DYIGDNTTVD YTLFESLCSK 51 KDVRNFKAWF LPIMYSIICF VGLLGNGLW LTYIYFKRLK TMTDTYLLNL 101 AVADILFLLT LPFWAYSAAK SWVFGVHFCK LIFAIYKMSF FSGMLLLLCI 151 SIDRYVAIVQ AVSAHRHRAR VLLISKLSCV GIWILATVLS IPELLYSDLQ 201 RSSSEQAMRC SLITEHVEAFITIQVAQMVI GFLVPLLAMS FCYLVIIRTL 251 LQARNFERNK AIKVIIAWV VFIVFQLPYN GWLAQTVAN FNITSSTCEL 301 SKQLNIAYDV TYSLACVRCC VNPFLYAFIG VKFRNDLFKL FKDLGCLSQE 351 QLRQWSSCRH IRRSSMSVEA ETTTTFSP

Ttttt Tttttt Aaaaa

The receptor for Fas ligand is Fas Apol CD95 apoptosis 1 , a member of the TNFR superfamily. There is 50 amino acid identity between mouse and human Fas. A 68-amino-acid portion of the signal-transducing domain significantly conserved in the Fas antigen as well as in the type I tumour necrosis factor receptor is required for apoptosis and is called the death domain DD . Fas has additional effects besides its apoptosis-inducing ability. On fibroblasts, Fas ligation can lead to proliferation or...