Info

• Mitochondrial extract (TL, TS, or S-100;

• [a-32P]pCp (New England Nuclear;

see Protocol 11)

3000 Ci/mimol, 10 mCi/ml)

• Cytosolic RNA substrate (500 pg/ml)

• DMSO

prepared as described in Protocol 12

• RNAguard (Pharmacia, #27-0815-01,

• 10 mM ATP

33 p/ml)

• 1.0 M MgCI2

• DE81 discs and 0.5 M sodium phosphate

• 0.25 M Hepes-KOH, pH 7.9

buffer, pH 6.8, with 0.5% sodium pyro

• 0.1 M DTT

phosphate (see Protocol 9)

Method

0 0

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