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Figure 74-9

2 4 8 16 32 64 128 Aldosterone concentration in test sample (ng/d)

Basic principles of the enzyme-linked immunosorbent assay (ELISA) for measuring the concentration of a hormone (H). ABi and AB2 are antibodies that recognize the hormone at different binding sites, and AB3 is an antibody that recognizes AB2. E is an enzyme linked to AB3 that catalyzes the formation of a colored fluorescent product (P) from a substrate (S). The amount of the product is measured using optical methods and is proportional to the amount of hormone in the well if there are excess antibodies in the well.

"Standard curve" for radioimmunoassay of aldosterone. (Courtesy Dr. Manis Smith.)

Basic principles of the enzyme-linked immunosorbent assay (ELISA) for measuring the concentration of a hormone (H). ABi and AB2 are antibodies that recognize the hormone at different binding sites, and AB3 is an antibody that recognizes AB2. E is an enzyme linked to AB3 that catalyzes the formation of a colored fluorescent product (P) from a substrate (S). The amount of the product is measured using optical methods and is proportional to the amount of hormone in the well if there are excess antibodies in the well.

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