References

Gashler, A. and Sukhatme, V., Early growth response protein 1 (Egr-1) prototype of a zinc-finger family of transcription factors, Prog. Nucl. Acid Res., 50, 191-224, 1995. 2. Khachigian, L.M. and Collins, T., Inducible expression of Egr-1-dependent genes a paradigm of transcriptional activation in vascular endothelium, Circ. Res., 81, 457-461, 1997. 3. Khachigian, L.M., Williams, A.J., and Collins, T., Interplay of Sp1 and Egr-1 in the proximal PDGF-A promoter in cultured vascular endothelial...

Noboru Fukuda

Second Department of Internal Medicine, and Division of Cell Regeneration and Transplantation Advanced Medical Research Center, Nihon University School of Medicine 1.1 Hammerhead 1.2 Designing 1.3 Delivery of 1.3.1 Exogenous 1.3.2 Endogenous 1.4 Chemical Modification of 1.5 Chimeric 1.7 Group I Intron 1.8 Therapeutic Applications of 1.8.1 Clinical Trials of Ribozymes 8 1.8.2 Cardiovascular 1.8.3 Delivery of Ribozymes by Drug-Eluting 1.8.4 Ribozyme Treatments for Progressive Renal Diseases 14...

Addition of New Chemical Functional Groups to DNA

Compared with proteins, nucleic acids possess a relatively modest number of chemical functional groups that can be brought to bear on catalysis. The large number of FIGURE 4.4 Precursor molecules comprising a typical library for the in vitro selection of an RNA-cleaving DNA enzyme. The black line corresponds to fixed and random DNA regions. The gray line corresponds to a fixed RNA region that serves as a substrate for potential RNA-cleaving DNA enzymes. The encircled B corresponds to a biotin...

Unresolved Issues In Odnbased Gene Therapy

ODN-based gene therapy still has many unsolved problems, such as the short halflife, low efficiency of uptake, and degradation by endocytosis and nucleases. Therefore, many groups are currently focusing on modifications of the gel approach using a catheter delivery system. Further modification of ODN pharmacokinetics will facilitate the potential clinical utility of the agents by 1. Allowing a shorter intraluminal incubation time to preserve organ perfusion 2. Prolonging the duration of...

About the Lead Authors

Banerjea, Ph.D. is a senior scientist at National Institute of Immunology, New Delhi, India, and is a member of National Academy of Sciences, India. He is involved in studying pathogenesis of several viruses, especially HIV. He is currently a Visiting Professor at Colorado State University, Colorado. He received his Bachelor's degree from Gorakhpur University, and a Master's degree from G.B. Pant University, U.P. He received his Ph.D. degree in Virology from Pune University. He was a...