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Electronics Repair Manuals Summary

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Interplay Between Thermodynamics Kinetics and Molecular Recognition

Schematic diagram for a hypothetical transition from the initial state, G , to two different solid forms A or B, with free energies GA and GB. Form A is more stable and less soluble than B. A transition from the initial state G to state A or B will depend on the energy barrier and according to this reaction pathway the height of the energy barrier for structure A, (GA G ) is greater than that for B, (GB G ). Because the rate of nucleation is related to the height of the energy barrier on the reaction path, B will nucleate at a faster rate than A even though the change in free energy is greater for A (GA G ) than for B (GB G ). From Rodriguez-Spong et al., (2004). Figure 11. Schematic diagram for a hypothetical transition from the initial state, G , to two different solid forms A or B, with free energies GA and GB. Form A is more stable and less soluble than B. A transition from the initial state G to state A or B will depend on the energy barrier and according to this...

Strategy to Introduce BiAllelic Mutations

The mouse BLM homolog, Blm, was modified by the insertion of a tetracycline cassette (tet cassette) in order to regulate Blm in a reversible manner the schematic diagram in Fig. 1 shows the modified Blm containing the tet cassette. The tet cassettes were inserted immediately upstream of the translational initiation sites of both alleles of Blm to generate Blmtet. Regulation of Blm expression was examined with use of a potent tetracycline analog, doxycycline (dox). Addition of dox in ESCs bearing Blmtet resulted in immediate reduction of Blm. In the absence of Blm, elevated sister chromatid exchange (SCE), a hallmark of BLM deficiency, was observed (Fig. 2). Lack of BLM also leads to an elevated rate of crossing over. To test the effect of transient loss of Blm on the cross over rate, we inserted a mutant Neo gene into the Fas ligand locus in Blmtet cells. High dosage of G418 ( 1 mg ml) selects for only cells bearing bi-allelic mutant Neo genes but not those bearing the mono-allelic...

Cellular promoters and RPEtargeting

Schematic diagram of CatD proximal promoter regions used in plasmid constructions. ERE estrogen responsive element, RARE retinoic acid response element, MLPE major late promoter element, DRE dioxin responsive element, GFP green fluorescent protein. Figure 37.1. Schematic diagram of CatD proximal promoter regions used in plasmid constructions. ERE estrogen responsive element, RARE retinoic acid response element, MLPE major late promoter element, DRE dioxin responsive element, GFP green fluorescent protein.

Asymmetric Enhancement Of Hippocampal Longterm Potentiation

Figure 2.4 (a) A schematic diagram of a coronal slice of the hippocampus showing the stimulation and recording sites. (c) A time line for in vitro electrophysiological recordings in CORT-treated and control slices. Note that the time delay to LTP induction was matched between the CORT and ACSF conditions, and the acute inhibitory effect was washed out with the perfusion of ACSF before induction. (b, d) Examples of population spike and EPSP LTP, respectively. Figure 2.4 (a) A schematic diagram of a coronal slice of the hippocampus showing the stimulation and recording sites. (c) A time line for in vitro electrophysiological recordings in CORT-treated and control slices. Note that the time delay to LTP induction was matched between the CORT and ACSF conditions, and the acute inhibitory effect was washed out with the perfusion of ACSF before induction. (b, d) Examples of population spike and EPSP LTP, respectively.

Evidence for TTXresistant action potentials in sensory nerve terminals

A schematic diagram of recording set-up and photomicrograph showing the location of the recording electrode (scale bar, 1 mm). B confocal micrograph of nerve terminals revealed with antibody to PGP 9.5 in the guinea-pig cornea. Most nerve terminals approach the surface of the epithelium at right angles and appear as single dots. C a single nerve terminal impulse (NTI) evoked by electrical stimulation of the ciliary nerves at the back of the eye. The upper part shows 50 overlaid traces recorded during a train of stimuli at 1 Hz and the lower part shows the average of these traces (SA, stimulation artefact). D frequency distribution of conduction velocities for all single units recorded. Used by permission 40 A schematic diagram of recording set-up and photomicrograph showing the location of the recording electrode (scale bar, 1 mm). B confocal micrograph of nerve terminals revealed with antibody to PGP 9.5 in the guinea-pig cornea. Most nerve terminals approach the surface of the...

Clathrin A Scaffold for Protein Coats

Schematic diagrams of clathrin, an AP complex, and clathrin-AP interactions at the membrane. A) The clathrin triskelion consists of three heavy chains (HC) and three light chains (LC). The HC is further subdivided into an amino-terminal globular domain (GD) linker (L) distal leg (DL) and a carboxy-terminal proximal leg (PL) domain, which joins with two other PL domains to form the vertex of three heavy chains. Triskelia assemble into a closed hexagonal lattice-work (here highly simplified), referred to as a clathrin cage or basket, which disassembles upon coat disassociation. The relative position of an individual triskelion in a clathrin cage is indicated. B) AP complexes consist of one 3-adaptin one y, a, 6, or e-adaptin, for AP-1-4, respectively one l-adaptin and one a-adaptin subunit. Relative positions of carboxyl-terminal Ear, Hinge, Trunk, and regions comprising amino-terminal Head domains of large adaptins, as well as -homology domain ( -HD) of l-adaptin, are...

PSCs guide regenerating I nerve terminals

Figure 5.7 A schematic diagram summarising the role of PSCs in the reinnervation of frog NMJs. Unlike mammals, nerve injury per se does not induce PSC process sprouting in the frog (a). Large numbers of PSC processess are seen after the arrival of regenerating nerves (b). As reinnervation progresses, regenerating nerve terminals grow along the preceding PSC processess (c). The result suggests that, following nerve injury in frog muscles, regenerating nerve terminals trigger PSC sprouting, and these PSC sprouts in turn guide regenerating nerve terminals. A colour version of this Figure is in the Plate section. Figure 5.7 A schematic diagram summarising the role of PSCs in the reinnervation of frog NMJs. Unlike mammals, nerve injury per se does not induce PSC process sprouting in the frog (a). Large numbers of PSC processess are seen after the arrival of regenerating nerves (b). As reinnervation progresses, regenerating nerve terminals grow along the preceding PSC processess (c). The...

Specific asthma drug treatment

Figure 13.2 (A) Schematic diagram of an asthmatic patient exhibiting significant residual flow at end expiration. (B) The oesophageal pressure (Poes), an estimate of intrapleural pressure, shows the degree of pressure change required to overcome intrinsic pressure (PEEPi) and initiate inspiratory flow. (C) A progressive increase in lung volume (breath stacking) occurs if expiratory time is insufficient to allow complete exhalation of the tidal volume. Figure 13.2 (A) Schematic diagram of an asthmatic patient exhibiting significant residual flow at end expiration. (B) The oesophageal pressure (Poes), an estimate of intrapleural pressure, shows the degree of pressure change required to overcome intrinsic pressure (PEEPi) and initiate inspiratory flow. (C) A progressive increase in lung volume (breath stacking) occurs if expiratory time is insufficient to allow complete exhalation of the tidal volume.

Functional Sites in GroEL Defined by Mutagenesis

Schematic diagram of the subunit arrangement in a hypothetical slice through the oligomer, showing the major functional sites, (a) GroEL, based on the crystal structure with bound substrate (shaded light to dark) as imaged by cryo-EM. A, apical domain I, intermediate domain E, equatorial domain. The apical domains form a ring of handlike structures with the substrate binding sites on the fingers protruding into the central channel. Sites of potential hinge rotations are indicated by black dots. The notch in the equatorial domain represents the ATP-binding cleft. The interaction across the equatorial plane is shown as pairs of wavy surfaces, (b) The folding complex GroEL-MDH-GroES-ATP, rotating the subunit domains and adding the GroES (dark gray) and substrate (shaded) densities according to cryo EM observations. Fig. 6. Schematic diagram of the subunit arrangement in a hypothetical slice through the oligomer, showing the major functional sites, (a) GroEL, based on the crystal...

Spatial And Pattern Selectivity In The Lateral Belt

Schematic diagram of dual auditory cortical pathways in primates representing auditory object pattern (what) processing in an antero-ventral projection and auditory space (where) processing in a postero-dorsal projection (modified and expanded from (Rauschecker, 1998) and (Rauschecker and Tian, 2000)). The auditory where-pathway is the main topic of the present chapter. Its projections are highlighted in solid lines participating cortical areas are marked with oblique lines. The antero-ventral pathway is shown in dashed lines. Areas that are not uniquely participating in either pathway are shown in dark blocks (primary auditory cortex, A1) or stippled (middle lateral belt area, ML). Prefrontal connections of the lateral belt areas are also shown directly on a lateral view of a rhesus monkey brain (from (Romanski et al., 1999)). Abbreviations MGd medial geniculate nucleus, dorsal division MGv medial geniculate nucleus, ventral division CM caudomedial area R rostral area CL...

Transmission and Processing of Signals in Neuronal Pools

Facilitation Neuronal Pools

Organization of Neurons for Relaying Signals. Figure 46-9 is a schematic diagram of several neurons in a neuronal pool, showing input fibers to the left and output fibers to the right. Each input fiber divides hundreds to thousands of times, providing a thousand or more terminal fibrils that spread into a large area in the pool to synapse with dendrites or cell bodies of the neurons in the pool. The dendrites usually also arborize and spread hundreds to thousands of micrometers in the pool.

Hybrid Multiscale Model of Solid Tumour Growth and Invasion Evolution and the Micro environment

Schematic diagram showing the key variables involved in solid tumour growth Tumour cells, extracellular matrix, matrix degrading enzyme and oxygen. The tumour contains a heterogeneous population of cells with varying degrees of aggressiveness. Figure 1. Schematic diagram showing the key variables involved in solid tumour growth Tumour cells, extracellular matrix, matrix degrading enzyme and oxygen. The tumour contains a heterogeneous population of cells with varying degrees of aggressiveness.

Where Do the Cancers Arise

Schematic diagram of various lineages that respond to specific cell damaging insults and therefore are likely founder cells for the tumours that develop subsequently. (1) The cells that normally respond to hepatocyte loss are the hepatocytes themselves (2) potential stem cells may reside in the canal of Hering and they or their progeny (oval cells HPCs) may give rise to most HCCs (3) the interlobular bile duct epithelia may give rise to CCs associated with fluke infection and (4) periductular cells are associated with experimental hepatocarcinogenesis associated with ethionine and a choline-deficient diet. Largely based on an idea by Stewart Sell. Fig. 3. Schematic diagram of various lineages that respond to specific cell damaging insults and therefore are likely founder cells for the tumours that develop subsequently. (1) The cells that normally respond to hepatocyte loss are the hepatocytes themselves (2) potential stem cells may reside in the canal of Hering and they or...

Hiv Rt Crystal Structures

Schematic diagram showing the overall structure of HIV-1 reverse transcriptase (RT). The protein chains are shown as ribbons and coils with the p66 subunit in light gray and the p51 subunit in dark gray. The double-stranded deoxyribonucleic acid in the structure of a catalytic complex (29) is drawn as spiral ladder with T and P marking the template and the primer, respectively, whereas the bound thymidine triphos-phate (dTTP) is drawn in ball-and-stick representation. The key residues of the polymerase and ribonuclease H (RNase H) active sites are indicated as black spheres. The gray spheres represent the sites of nucleoside analog RT inhibitor (NRTI)-resis-tance mutations. The nevirapine molecule shown as a black space-filling model marks the non-nucleoside RT inhibitor (NNRTI) site. Fig. 1. Schematic diagram showing the overall structure of HIV-1 reverse transcriptase (RT). The protein chains are shown as ribbons and coils with the p66 subunit in light gray and the p51...

Application of NIR to fruit and vegetables

Although the penetrating power of the true NIR region is limited, that of the near visible is better. This has recently been used by a team which has developed a new method to allow the interior of fruit and vegetables to be assessed more accurately. The method is called the V-method and uses a system of transmitted and reflected light to 'virtually peel' fruit or vegetables where skin or peel serves to render the contents 'invisible' (Krivoshiev et al, 2000)). The method may be understood with reference to Fig. 7.3. Here the composite nature of the transmitted light passing through a schematic diagram of a fruit or vegetable may be seen. The incident energy interacts with the peel or skin on both sides of the object as well as the fleshy interior. By using

Monoamine Transporters

Schematic diagram of the catecholamine transporters showing the 12 transmembrane, hydrophobic domains and the -NH2 and -COOH termini. Targets for specific binding ligands are thought to be within regions indicated by the solid bars. Based on refs. 18, 30, and 34. Fig. 10.1. Schematic diagram of the catecholamine transporters showing the 12 transmembrane, hydrophobic domains and the -NH2 and -COOH termini. Targets for specific binding ligands are thought to be within regions indicated by the solid bars. Based on refs. 18, 30, and 34.

Preclinical Development and Validation of Liposome Entrapped raf Antisense Oligonucleotide

A schematic diagram of LErafAON formulation is shown in Fig. 4A. The rafAON is a 15-mer ASO with phosphorothioate linkage limited to one base at 5'- and 3'-ends (30). The rafAON sequence is targeted against the translation initiation region of c-raf-1 mRNA. The liposome formulation consists of a mixture of a cationic lipid (dimethyldioctadecyl ammonium bromide), egg phos-phatidylchloline, and cholesterol in a molar ratio of 1 3.2 1.6. The rafAON to lipid ratio is 1 15 (w w). The particle size was found to be approx 500 nm and entrapment efficiency was > 85 . The LErafAON formulation was stable at room temperature for at least 1 wk as shown by the presence of 15-mer rafAON (Fig. 4B).

Outline of the Production System and Factors Affecting Production

Algae can be produced either in open ponds or in closed bioreactors. The latter are believed to be more productive but a recent side-by-side comparison study has shown that there is virtually no difference in aerial productivity using the two systems (Paola Pedroni, personal communication). Since there is very little, if any, commercial production of A. platensis or A. maxima in closed bioreactors, the following discussion deals with open-pond systems only. A schematic diagram of a typical production system is given in Figure 1.3.

Destruction

Schematic diagram of major issues addressed by this report. Chlorofluorocarbons (CFCs), halons and hydrochlorofluorocarbons (HCFCs) contribute to ozone depletion and climate change, while hydrofluorocarbons (HFCs) and perfluorocarbons (PFCs) contribute only to climate change and are among possible non-ozone depleting alternatives for ODSs. Red denotes gases included under the Montreal Protocol and its amendments and adjustments4 while green denotes those included under the UNFCCC and its Kyoto Protocol. Options considered in this report for reducing halocarbon emissions include improved containment, recovery, recycling, destruction of byproducts and existing banks5, and use of alternative processes, or substances with reduced or negligible global warming potentials.

Prokaryote

Fig. 7.1. (a) A schematic diagram of a eukaryote. (b) Typical prokaryotic and eukaryotic cells, based on electron microscopy. Not every prokaryote or eukaryote has every feature shown here. (From Margulis and Schwartz (1998). Five Kingdoms. Copyright W. H. Freeman and Company, New York. Reproduced with permission.)

Basic principles

Schematic diagram of an optical trap. A high-numerical-aperture objective lens forms a focus in which a refractile particle is trapped in 3D with forces of typically up to 100 pN. The condenser collects the transmitted light and a quadrant photodiode serves as displacement and force detector.

Anatomy Histology

Motor Unit Action Potential Muap

Schematic diagram of the connective tissue sheaths of muscle. Muscle fibers are bundled into fascicles bordered by perimysial connective tissue. From DeGirolami and Smith, 1982 with permission. Fig. 1. Schematic diagram of the connective tissue sheaths of muscle. Muscle fibers are bundled into fascicles bordered by perimysial connective tissue. From DeGirolami and Smith, 1982 with permission.

Extraocular Muscles

Figure.9-2 represents a schematic diagram of the orbits viewed from above, with the eyes looking to the right. The superior rectus and superior oblique muscles are shown with their insertions on the top of the globes--the superior rectus muscle elevates the globe and the superior oblique muscle depresses the globe. The inferior rectus and oblique muscles insert at homologous sites on the bottom of the globe, and the horizontal vectors of pull are the same, except the inferior rectus muscle depresses the globe while the inferior oblique muscle elevates it. The adducted position of the left eye places the superior oblique in a position to depress the eye as a primary and direct action (see also Figure 9-2 B and I blei9-2 ). The abducted position of the right eye puts the right superior rectus in position to elevate the right eye as a primary and direct action. The direction of pull of the left superior rectus muscle on the adducted left eye puts it in position to intort the eye as a...

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