Analytical methods for steviol glycosides in stevia sweeteners

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According to the Voluntary Specifications of Non-chemically Synthesized Food Additives (Second Edition 1993), the quantitative analysis oftotal steviol glycosides in stevia extracts is performed using high-performance liquid chromatography (HPLC) on an NH2 column (normal-phase mode), in the following manner. About 60 to 120 mg of the sample is accurately weighed and dissolved in 80 (v v) acetonitrile to make 100ml of a test solution. About 50 mg each of stevioside and rebaudioside A, dried...